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PGD2 (prostaglandin D2) RIA kit

Intended Use:

Like other prostaglandins, PGD2 is formed from prostaglandin endoperoxides which are produced from arachidonic acid on the action of cyclooxygenase enzyme. PGD2 has various biological actions, e.g. decreases thrombocyte aggregation, it is a potent broncho-constrictor in dog, it has a potenciating effect on the inflammatory response to histamine, it increases the chemotactic activity of eosinophil granulocytes, etc. One of its prominent functions seems to be its suggested role as a neuromodulator in the central nervous system. PGD2 was also reported to have a significantly lower concentration in a highly metastatic mouse melanoma cell line than in a less metastatic one. On this basis PGD2 was suggested to decrease tumor metastasis and progression. Later its direct inhibitory action on malignant tumour cell proliferation was proved in various cell lines. The instability of PGD2 may account for the difficulties encountered in the course of its radioimmunological determination, including the problems with the production of specific antisera with proper binding ability as well as the low sensitivity of assay.

Assay Characteristics:

Assay type:   RIA
Isotope: 3H
Separation: Dextran-coated charcoal
Sample type: human plasma
Sample preparation: solid-phase extraction
Sample volume: 100 µl
Sample capacity: 42 unknowns
Incubation: Overnight at +2-4°C, 18-20 hours
Time: one working day
Hands-on time: 4 hours

Special Advantages:

  • Highly increased sensitivity
  • Low stability
  • Robust and precise assay protocol
  • Protocol for sample preparation
  • Wide calibration range
  • High specificity
  • High reproducibility
  • Availability: from stock  (Not available in Austria, Germany and UK)

    Pack Contents:

  • [3H] – prostaglandin D2 Tracer, in organic solvent mixture
  • prostaglandin D2 Standard, lyophilised
  • prostaglandin D2 rabbit Antiserum, lyophilised
  • Assay Buffer concentrate
  • Charcoal suspension, ready to use
  • Full instructions booklet
  • Assay Parameters:

    Measuring range:  1.2-100 pg/tube
    Sensitivity: ~ 1.5 pg/tube  
    Typical IC50 value: 10-20 pg/tube  
    Specificity: See cross reactions (at 50% displacement) below  
      PGF1 0.004
      Prostaglandin E2 0.020
      Prostaglandin J2 7.000
      Prostaglandin A2 0.010
      Prostaglandin E1 0.003
      13,14-dihydro-15-keto-PGF 0.020
      13,14-dihydro-6,15-diketo-PGFα 0.020
      6-keto-PGE1 0.001
      TxB2 0.030
      Arachidonic acid 0.002
      PGF 0.040

     

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